ELECTROSTIMULATED UPTAKE OF DNA BY LIPOSOMES

被引：51

作者：

CHERNOMORDIK, LV ^{[1
]}

SOKOLOV, AV ^{[1
]}

BUDKER, VG ^{[1
]}

机构：

[1] ACAD SCI USSR,INST BIOORGAN CHEM,NOVOSIBIRSK,USSR

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1024卷 / 01期

关键词：

DNA transfer; Electrostimulation; Liposome;

D O I：

10.1016/0005-2736(90)90222-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High molecular mass DNA was efficiently taken up by large unilamellar vesicles exposed to a short pulse of electric field (0.1-1 ms) with an intensity as high as 12.5 kV/cm. The efficiency of uptake increased significantly in presence of Mg2+ ions and was approximately 0.6 and 1.5 μg of DNA per μmol of lipid for T7 DNA and plasmid pBR 322, respectively. The results presented indicated that DNA was taken up as a result of the electrostimulated formation of endosome-like vesicles rather than via field-induced membrane pores. © 1990.

引用

页码：179 / 183

页数：5

共 19 条

[1] BUDKER VG, 1987, BIOL MEMBRANY, V4, P1201
[2] BUDKER VG, 1987, BIOL MEMBRANY, V4, P55
[3] INTERACTION OF POLYNUCLEOTIDES WITH NATURAL AND MODEL MEMBRANES
BUDKER, VG
GODOVIKOV, AA
NAUMOVA, LP
SLEPNEVA, IA
[J]. NUCLEIC ACIDS RESEARCH, 1980, 8 (11) : 2499 - 2515
[4] THE ELECTRICAL BREAKDOWN OF CELL AND LIPID-MEMBRANES - THE SIMILARITY OF PHENOMENOLOGIES
CHERNOMORDIK, LV
SUKHAREV, SI
POPOV, SV
PASTUSHENKO, VF
SOKIRKO, AV
ABIDOR, IG
CHIZMADZHEV, YA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 902 (03) : 360 - 373
[5] ELECTROPORATION FOR THE EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS WITH DNA
CHU, G
HAYAKAWA, H
BERG, P
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (03) : 1311 - 1326
[6] ELECTROSTIMULATED TRANSFORMATION OF ESCHERICHIA-COLI-CELLS PRETREATED BY EDTA SOLUTION
CYMBALYUK, ES
CHERNOMORDIK, LV
BROUDE, NE
CHIZMADZHEV, YA
[J]. FEBS LETTERS, 1988, 234 (01) : 203 - 207
[7] PHYSICOCHEMICAL CHARACTERIZATION OF LARGE UNILAMELLAR PHOSPHOLIPID-VESICLES PREPARED BY REVERSE-PHASE EVAPORATION
DUZGUNES, N
WILSCHUT, J
HONG, K
FRALEY, R
PERRY, C
FRIEND, DS
JAMES, TL
PAPAHADJOPOULOS, D
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 732 (01) : 289 - 299
[8] REVERSIBLE PLASMA-MEMBRANE ULTRASTRUCTURAL-CHANGES CORRELATED WITH ELECTROPERMEABILIZATION IN CHINESE-HAMSTER OVARY CELLS
ESCANDEGERAUD, ML
ROLS, MP
DUPONT, MA
GAS, N
TEISSIE, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 939 (02) : 247 - 259
[9] HARBICH W, 1979, Z NATURFORSCH A, V34, P1063
[10] ROLE OF LIPID PHASE SEPARATIONS AND MEMBRANE HYDRATION IN PHOSPHOLIPID VESICLE FUSION
HOEKSTRA, D
[J]. BIOCHEMISTRY, 1982, 21 (12) : 2833 - 2840

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