CLONING AND CHARACTERISTICS OF A POSITIVE REGULATORY GENE, THI2 (PHO6), OF THIAMIN BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE

被引：44

作者：

NISHIMURA, H ^{[1
]}

KAWASAKI, Y ^{[1
]}

KANEKO, Y ^{[1
]}

NOSAKA, K ^{[1
]}

IWASHIMA, A ^{[1
]}

机构：

[1] INST FERMENTAT,YODOGAWA KU,OSAKA 532,JAPAN

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

CLONING; POSITIVE REGULATORY GENE; THIAMIN METABOLISM; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(92)80349-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A thi2(pho6) mutant of Saccharomyces cerevisiae, defective in the expression of structural genes for thiamin-repressible acid phosphatase and enzymes involved in thiamin biosynthesis, was found to retain sufficient thiamin transport activity. The transport activity was repressed by thiamin in growth medium. We isolated from a S. cerevisiae genomic library two hybrid plasmids, pTSR1 and pTSR2, containing 10.2- and 12.0-kilobase (kb) DNA fragments, respectively, which complement the thi2(pho6) mutation of S. cerevisiae. This gene was localized on a 6.0-kb ClaI-ClaI fragment in the subclone pTSR3. Complementation of the enzyme activities for thiamin metabolism in the thi2(pho6) mutant transformed by some plasmids with the THI2(PHO6) gene was also examined.

引用

页码：155 / 158

页数：4

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