DIRECT AND QUANTITATIVE DETECTION OF HIV-1 RNA IN HUMAN PLASMA WITH A BRANCHED DNA SIGNAL AMPLIFICATION ASSAY

被引：63

作者：

URDEA, MS ^{[1
]}

WILBER, JC ^{[1
]}

YEGHIAZARIAN, T ^{[1
]}

TODD, JA ^{[1
]}

KERN, DG ^{[1
]}

FONG, SJ ^{[1
]}

BESEMER, D ^{[1
]}

HOO, B ^{[1
]}

SHERIDAN, PJ ^{[1
]}

KOKKA, R ^{[1
]}

NEUWALD, P ^{[1
]}

PACHL, CA ^{[1
]}

机构：

[1] CHIRON CORP,NUCLE ACID SYSTEMS,EMERYVILLE,CA 94608

来源：

AIDS | 1993年 / 7卷

关键词：

HIV RNA; BRANCHED DNA SIGNAL AMPLIFICATION; REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTION; QUANTITATION;

D O I：

10.1097/00002030-199311002-00004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim: To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma. Method: Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were used to measure the viral load. Results: In one sample the viral load by polymerase chain reaction ranged from undetectable to 1.9 x 10(5) copies/ml, and the branched DNA results ranged from 2.6 x 10(4) to 4.2 x 10(4) HIV RNA equivalents/ml. In the other sample the corresponding figures were 6.3 x 10(4) to 5.5 x 10(5) copies/ml and 5.7 x 10(4) to 7.5 x 10(4) HIV RNA equivalents/ml. Conclusion: In contrast to reverse-transcriptase polymerase chain reaction the branched DNA signal amplification assay does not require a separate extraction step or enzymatic amplification of the target. Therefore this measurement is less affected by the sample matrix and the signal generated is directly proportional to the viral load.

引用

页码：S11 / S14

页数：4

共 7 条

[1] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[2] EHRLICH GD, 1990, PCR PROTOCOL GUIDE M, P333
[3] AVOIDING FALSE POSITIVES WITH PCR
KWOK, S
HIGUCHI, R
[J]. NATURE, 1989, 339 (6221) : 237 - 238
[4] PACHL C, 1993, APR KEYST S FRONT HI
[5] PACHL C, 1993, 9 INT C AIDS BERL
[6] PACHL CA, 1992, 32 INT C ANT AG CHEM
[7] URDEA MS, 1991, NUCLEIC ACIDS RES S, V24

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