DETERMINING RELEASE RATES OF 3-METHYL-2-CYCLOHEXEN-1-ONE ANTIAGGREGATION PHEROMONE OF Dendroctonus pseudotsugae (COLEOPTERA : SCOLYTIDAE)
被引:1
作者:
Look, Melvin
论文数: 0引用数: 0
h-index: 0
机构:
USDA, US Forest Serv, Pacific Southwest Forest & Range Expt Stn, Berkeley, CA 94710 USAUSDA, US Forest Serv, Pacific Southwest Forest & Range Expt Stn, Berkeley, CA 94710 USA
Look, Melvin
[1
]
机构:
[1] USDA, US Forest Serv, Pacific Southwest Forest & Range Expt Stn, Berkeley, CA 94710 USA
antiaggregation pheromone;
controlled-release formulation;
high-pressure liquid chromatography;
Douglas fir beetle;
Dendroctonus pseudotsugae;
D O I:
10.1007/BF00988813
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
A method of analysis using high-pressure liquid chromatography (HPLC) with an ultraviolet detector was developed for determining release rates of the antiaggregation pheromone of Dendroctonus pseudotsugae Hopkins, 3-methyl-2-cyclohexen-1-one (MCH), in controlled-release formulations. The pheromone released by the formulations was swept from the test apparatus, trapped on Porapak-QS packing, and eluted with solvent. Aliquots of the eluate were analyzed by liquid chromatography. Release rates of 5-400 mu g MCH/hr per g formulation were determined from 5-g samples of the controlled-release formulations.