DETERMINING RELEASE RATES OF 3-METHYL-2-CYCLOHEXEN-1-ONE ANTIAGGREGATION PHEROMONE OF Dendroctonus pseudotsugae (COLEOPTERA : SCOLYTIDAE)

被引:1
作者
Look, Melvin [1 ]
机构
[1] USDA, US Forest Serv, Pacific Southwest Forest & Range Expt Stn, Berkeley, CA 94710 USA
关键词
antiaggregation pheromone; controlled-release formulation; high-pressure liquid chromatography; Douglas fir beetle; Dendroctonus pseudotsugae;
D O I
10.1007/BF00988813
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method of analysis using high-pressure liquid chromatography (HPLC) with an ultraviolet detector was developed for determining release rates of the antiaggregation pheromone of Dendroctonus pseudotsugae Hopkins, 3-methyl-2-cyclohexen-1-one (MCH), in controlled-release formulations. The pheromone released by the formulations was swept from the test apparatus, trapped on Porapak-QS packing, and eluted with solvent. Aliquots of the eluate were analyzed by liquid chromatography. Release rates of 5-400 mu g MCH/hr per g formulation were determined from 5-g samples of the controlled-release formulations.
引用
收藏
页码:481 / 486
页数:6
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