CORRECTIONS FOR INSTRUMENTAL ERRORS IN MEASUREMENT OF FLUORESCENCE AND POLARIZATION OF FLUORESCENCE

Calculations have been made to allow corrections for instrumental errors in the measurement of fluorescence and polarization coefficient. These errors are due to differences in transmittivities of the instruments for the horizontal and vertical components of the light. The relative error made in the quantum efficiency determination can be as large as 12%. When natural light is used for polarization measurement the relative error can be 15%. Special attention has been given to the case in which polarization measurements are used for measurements of binding of small molecules to macromolecules. © 1969.