FIXATION OF PROTEINS BY OSMIUM TETROXIDE POTASSIUM DICHROMATE AND POTASSIUM PERMANGANATE - MODEL EXPERIMENTS WITH BOVINE SERUM ALBUMIN AND BOVINE GAMMA-GLOBULIN

被引：29

作者：

HOPWOOD, D

机构：

[1] Department of Human Morphology, University of Nottingham

来源：

HISTOCHEMIE | 1969年 / 18卷 / 03期

关键词：

D O I：

10.1007/BF00306171

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The crosslinking abilities of osmium tetroxide, potassium dichromate and potassium permanganate towards bovine serum albumin and bovine γ-globulin were investigated by chromatography with Sephadex G-200. Osmium tetroxide had a moderate crosslinking ability towards these proteins, the others had little or none. Chromatography with Sephadex G-50 permitted the oxidative cleavage of the proteins by these oxidative fixation agents to be studied. Potassium permanganate caused much fragmentation of the proteins and destruction of the tyrosine and tryptophan residues. Osmium tetroxide and potassium dichromate caused only a small amount of protein cleavage. These results were corroborated by polyacrylamide gel electrophoresis and viscosimetric studies. The significance of the results for tissue fixation is discussed. © 1969 Springer-Verlag.

引用

页码：250 / &

共 37 条

[1] OSMIUM TETROXIDE AS A HISTOCHEMICAL AND HISTOLOLOGICAL REAGENT [J].