STUDIES ON DEBRANCHING ENZYMES .7. PARTIAL-PURIFICATION AND PROPERTIES OF A BACTERIAL ISOAMYLASE

被引：18

作者：

EVANS, RM

MANNERS, DJ

STARK, JR

机构：

[1] Department of Brewing and Biological Sciences, Heriot-Watt University, Edinburgh

来源：

CARBOHYDRATE RESEARCH | 1979年 / 76卷 / NOV期

关键词：

D O I：

10.1016/0008-6215(79)80019-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isoamylase was prepared by affinity chromatography of a commercial enzyme-preparation from a strain of Cytophaga (also known as a Flavobacterium or Polyangium). The enzyme was not very stable, but the stability could be improved by Ca ions. The enzyme had a very low but significant activity on pullulan and on .alpha.-dextrins having maltosyl side-chains. This observation, which is contrary to previous reports, was related to the specificity of isoamylase and other bacterial debranching-enzymes.

引用

页码：203 / 213

页数：11

共 28 条

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ABDULLAH M, 1966, CEREAL CHEM, V43, P111

[2]

BAILEY RW, 1967, 7TH INT C BIOCH TOK

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