GENE HOMOGENEITY FOR AMINOGLYCOSIDE-MODIFYING ENZYMES IN GRAM-POSITIVE COCCI

被引:77
作者
OUNISSI, H
DERLOT, E
CARLIER, C
COURVALIN, P
机构
[1] INST PASTEUR,CNRS,UNITE AGENTS ANTIBACTERIENS 271,F-75724 PARIS 15,FRANCE
[2] UNIV CALIF SAN DIEGO,CTR MOLEC GENET,LA JOLLA,CA 92093
关键词
D O I
10.1128/AAC.34.11.2164
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Aminoglycoside-resistant strains of Staphylococcus and Enterococcus, approximately 500 of each, were screened by dot blot hybridization for the presence of genes encoding aminoglycoside-modifying enzymes. The MICs of various aminoglycosides for the strains were determined, and the enzyme contents of the cells were inferred from the resistance phenotypes. The agreements (in percent) of the hybridization results with the deduced enzyme contents for Staphylococcus and Enterococcus species were, respectively, 80 and 87.6 for ANT(6) (aminoglycoside nucleotidyltransferase), 99.8 and 100 for both APH(3') (aminoglycoside phosphotransferase) and APH(2'')-AAC(6') (aminoglycoside acetyltransferase), and 100 and 100 for ANT(4'). The weak correlation obtained with the probe for ANT(6) was due to the fact that gram-positive cocci can also be streptomycin resistant by synthesis of APH(3'') or ANT (3'')(9) and by ribosomal mutation. The remaining probes appeared to be specific: they hybridized with all the resistant clinical isolates but not with the susceptible strains. These results indicate that, except for streptomycin, nucleic acid hybridization is a valid approach for the detection and characterization of aminoglycoside resistance in gram-positive cocci.
引用
收藏
页码:2164 / 2168
页数:5
相关论文
共 39 条
[31]   TRANSFER OF STREPTOCOCCUS-FAECALIS AND STREPTOCOCCUS-FAECIUM TO THE GENUS ENTEROCOCCUS NOM REV AS ENTEROCOCCUS-FAECALIS COMB-NOV AND ENTEROCOCCUS-FAECIUM COMB-NOV [J].
SCHLEIFER, KH ;
KILPPERBALZ, R .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1984, 34 (01) :31-34
[32]   COMPARISON OF AMINOGLYCOSIDE RESISTANCE PATTERNS IN JAPAN, FORMOSA, AND KOREA, CHILE, AND THE UNITED-STATES [J].
SHIMIZU, K ;
KUMADA, T ;
HSIEH, WC ;
CHUNG, HY ;
CHONG, Y ;
HARE, RS ;
MILLER, GH ;
SABATELLI, FJ ;
HOWARD, J .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 28 (02) :282-288
[33]  
SOUSSY CJ, 1979, NOUV PRESSE MED, V8, P3413
[34]  
STEERS EDWARD, 1959, ANTIBIOT AND CHEMOTHER, V9, P307
[35]   ANTAGONISTIC EFFECT OF PENICILLIN-AMIKACIN COMBINATIONS AGAINST ENTEROCOCCI [J].
THAUVIN, C ;
ELIOPOULOS, GM ;
WENNERSTEN, C ;
MOELLERING, RC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 28 (01) :78-83
[36]   NUCLEOTIDE-SEQUENCE OF THE STREPTOCOCCUS-FAECALIS PLASMID GENE ENCODING THE 3'5''-AMINOGLYCOSIDE PHOSPHOTRANSFERASE TYPE-III [J].
TRIEUCUOT, P ;
COURVALIN, P .
GENE, 1983, 23 (03) :331-341
[37]   THE PUC PLASMIDS, AN M13MP7-DERIVED SYSTEM FOR INSERTION MUTAGENESIS AND SEQUENCING WITH SYNTHETIC UNIVERSAL PRIMERS [J].
VIEIRA, J ;
MESSING, J .
GENE, 1982, 19 (03) :259-268
[38]  
Wennersten C, 1980, CURRENT CHEMOTHERAPY, V1, P710
[39]   OCCURRENCE OF THE CAMPYLOBACTER RESISTANCE GENE TETO IN ENTEROCOCCUS AND STREPTOCOCCUS SPP [J].
ZILHAO, R ;
PAPADOPOULOU, B ;
COURVALIN, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (12) :1793-1796