MEASUREMENT OF IGM RESPONSES TO A SUBUNIT INFLUENZA-A VACCINE BY SUCROSE-GRADIENT CENTRIFUGATION AND MEMBRANE-FILTRATION ENZYME IMMUNOASSAYS

被引:3
作者
TANNOCK, GA
BRYCE, DA
BARNETT, GR
HAMPSON, AW
HENSLEY, MJ
SAUNDERS, NA
机构
[1] NEWCASTLE MATER MISERICORDIAE HOSP, DEPT MICROBIOL, WARATAH, NSW 2298, AUSTRALIA
[2] COMMONWEALTH SERUM LABS, PARKVILLE, VIC 3052, AUSTRALIA
关键词
D O I
10.1016/1045-1056(91)90019-G
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
IgM responses to a deoxycholate-split influenza vaccine containing the surface antigens of the H3N2 virus A/Philippines/2/82 were studied in five volunteers, three of whom were seronegative by haemagglutination inhibition (HI) tests. Responses were measured by a sucrose-gradient centrifugation technique, in which IgM-specific HI activity was computed as a proportion of total IgM and IgG-specific HI activity, and by a membrane filtration-enzyme immunoassay (MF-EIA). Responses weeks, and the IgM induced was 5-40% of total HI-specific activity. The response was also observed in the presence of low levels of pre-existing antibody. Levels of IgM antibody, when measured by MF-EIA, could be easily detected in two of the volunteers, while those of two others were very low and there was no response in a fifth. No biphasic virus-specific response to vaccination, involving first IgM and then IgG, could be measured by either technique. From these studies, the sucrose-gradient fractionation technique appears to be the more sensitive procedure. © 1991.
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页码:17 / 21
页数:5
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