SPECIFIC INTERACTION OF INITIATION-FACTOR IF2 OF ESCHERICHIA-COLI WITH FORMYLMETHIONYL-TRANSFER RNAFMET

被引：57

作者：

PETERSEN, HU ^{[1
]}

ROLL, T ^{[1
]}

GRUNBERGMANAGO, M ^{[1
]}

CLARK, BFC ^{[1
]}

机构：

[1] INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91989-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of E.coli initiation factor IF2 with formylmethionyl-tRNAfMet has been studied by measuring the inhibition by IF2 of the spontaneous deaminoacylation of the charged tRNA. We find that IF2 protects fMet-tRNAfMet against spontaneous deacylation. The formylation is an absolute requirement for this protection and no effect of GTP was found. The association constant for IF2 binding to fMet-tRNAfMet at 37°C and physiological ionic conditions was estimated at about 106 M-1. © 1979.

引用

页码：1068 / 1074

页数：7

共 14 条

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