THE USE OF HPLC PROTEIN PROFILES IN FISH SPECIES IDENTIFICATION

被引：54

作者：

ARMSTRONG, SG ^{[1
]}

LEACH, DN ^{[1
]}

WYLLIE, SG ^{[1
]}

机构：

[1] UNIV WESTERN SYDNEY,CTR BIOSTRUCT & BIOMOLEC RES,RICHMOND,NSW 2753,AUSTRALIA

来源：

FOOD CHEMISTRY | 1992年 / 44卷 / 02期

关键词：

D O I：

10.1016/0308-8146(92)90328-Y

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method for the rapid identification of fish species by high performance liquid chromatographic (HPLC) analysis of water-soluble sarcoplasmic protein extracts is described. The method relies on the visual comparison of a star-symbol plot constructed from the sample's HPLC profile with those obtained from the mean profile data of morphologically identified species. A library of plots is presented for 15 common edible marine fish species from eastern Australian waters, but any suitable data can be used for star construction. The data presented are applicable to the identification of samples from any season or location, whether they are raw, gamma-irradiated, or dried with infra-red radiation. The procedure involves a single 10 min extraction followed by a 60 min analysis, without the use of an internal standard.

引用

页码：147 / 155

页数：9

共 7 条

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