MOLECULAR-CLONING AND EXPRESSION OF THE MOUSE 105-KDA GELATINASE CDNA

被引：95

作者：

TANAKA, H ^{[1
]}

HOJO, K ^{[1
]}

YOSHIDA, H ^{[1
]}

YOSHIOKA, T ^{[1
]}

SUGITA, K ^{[1
]}

机构：

[1] SHIONOGI & CO LTD,SHIONOGI RES LAB,DEPT MICROBIOL,DIV ONCOL,4-12,5-CHOME,FUKUSHIMA KU,OSAKA 553,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have detected a potent gelatinolytic activity in the culture supernatant of a metastatic tumor line, SN-H, derived from a murine squamous cell carcinoma. The relative molecular weight of the gelatinase was estimated as 105-kDa by gelatin zymography. We have cloned the cDNA of this gelatinase and the 3160-bp sequence has been determined. From the translated amino acid sequence, the positions of the cysteine residues and the functional domain structure are highly homologous to the human 92-kDa gelatinase. The nucleotide and amino acid sequence homology between these two cDNAs are 75% and 72%, respectively. Transfection of the cDNA in an expression vector resulted in production of the 105-kDa gelatinase, thus confirming that this cDNA is functional. © 1993 Academic Press, Inc.

引用

页码：732 / 740

页数：9

共 22 条

[1] RAS ONCOGENE MEDIATED INDUCTION OF A 92KDA METALLOPROTEINASE - STRONG CORRELATION WITH THE MALIGNANT PHENOTYPE [J].