HETEROGENEITY OF PROTEIN-KINASE-C ACTIVITY IN HUMAN U-373 AND G-26 MICE GLIOMA-CELLS

被引:3
作者
ACEVEDODUNCAN, M
ZHANG, R
机构
[1] UNIV S FLORIDA,JAMES A HALEY VET HOSP,COLL MED,DEPT RADIOL,DIV RADIAT ONCOL,TAMPA,FL
[2] H LEE MOFFIT CANC CTR & RES INST,TAMPA,FL
关键词
D O I
10.1006/bbrc.1994.2639
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinase C (PKC) enzyme activity in a mouse glioma cell line G-26 and a human glioma cell line U-373 were compared at similar cell confluency in-vitro to establish if a G-26 in-vivo mouse model would be useful to examine the role of PKC inhibitors in controlling human glioma growth in-vivo. Original crude cytosolic and membrane PKC fractions of both mouse glioma G-26 and human glioma U-373 cells did not display significant PKC activity compared to partially purified PKC. Partial purification of mouse glioma G-26 and U-373 cytosolic and membrane fractions showed different cytosolic and membrane PKC activity profiles. Total PKC activity was higher (rho 0.0001) in human glioma U-373 (7840 picomoles/mg/min) than in mouse glioma G-26 cells (2890 picomoles/mg/min). Thus, results from trials using nude mice human glioma xenografts may be more valid than those obtained from a G-26 in-vivo mouse model for studying the effects of therapeutic drugs on PKC isozymes. (C) 1994 Academic Press, Inc.
引用
收藏
页码:127 / 134
页数:8
相关论文
共 15 条
[1]   IMMUNOLOGICAL EVIDENCE THAT INSULIN ACTIVATES PROTEIN KINASE-C IN BC3H-1 MYOCYTES [J].
ACEVEDODUNCAN, M ;
COOPER, DR ;
STANDAERT, ML ;
FARESE, RV .
FEBS LETTERS, 1989, 244 (01) :174-176
[2]  
AKINAGA S, 1991, CANCER RES, V51, P4888
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   ENHANCED PROTEIN-KINASE-C ACTIVITY CORRELATES WITH THE GROWTH-RATE OF MALIGNANT GLIOMAS INVITRO [J].
COULDWELL, WT ;
UHM, JH ;
ANTEL, JP ;
YONG, VW .
NEUROSURGERY, 1991, 29 (06) :880-887
[5]   ACTIVATION OF PROTEIN-KINASE IN THE BOVINE CORPUS-LUTEUM BY PHOSPHOLIPID AND CA-2+ [J].
DAVIS, JS ;
CLARK, MR .
BIOCHEMICAL JOURNAL, 1983, 214 (02) :569-574
[6]   RAPID FILTRATION ASSAYS FOR PROTEIN-KINASE-C ACTIVITY AND PHORBOL ESTER BINDING USING MULTIWELL PLATES WITH FITTED FILTRATION DISKS [J].
GOPALAKRISHNA, R ;
CHEN, ZH ;
GUNDIMEDA, U ;
WILSON, JC ;
ANDERSON, WB .
ANALYTICAL BIOCHEMISTRY, 1992, 206 (01) :24-35
[7]   RETINOIDS INHIBIT THE OXIDATIVE MODIFICATION OF PROTEIN-KINASE-C INDUCED BY OXIDANT TUMOR PROMOTERS [J].
GUNDIMEDA, U ;
HARA, SK ;
ANDERSON, WB ;
GOPALAKRISHNA, R .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (01) :526-530
[8]   A DERIVATIVE OF STAUROSPORINE (CGP-41-251) SHOWS SELECTIVITY FOR PROTEIN-KINASE C INHIBITION AND INVITRO ANTI-PROLIFERATIVE AS WELL AS INVIVO ANTI-TUMOR ACTIVITY [J].
MEYER, T ;
REGENASS, U ;
FABBRO, D ;
ALTERI, E ;
ROSEL, J ;
MULLER, M ;
CARAVATTI, G ;
MATTER, A .
INTERNATIONAL JOURNAL OF CANCER, 1989, 43 (05) :851-856
[9]  
PONTEN J, 1968, ACTA PATHOL MIC SC, V74, P465
[10]   IMMUNOHISTOCHEMICAL DETERMINATION OF PROTEIN KINASE-C EXPRESSION AND PROLIFERATIVE ACTIVITY IN HUMAN-BRAIN TUMORS [J].
REIFENBERGER, G ;
DECKERT, M ;
WECHSLER, W .
ACTA NEUROPATHOLOGICA, 1989, 78 (02) :166-175