ANTIOXIDANT, PROBUCOL, CAN INHIBIT THE GENERATION OF HYDROGEN-PEROXIDE IN ISLET CELLS INDUCED BY MACROPHAGES AND PREVENT ISLET-CELL DESTRUCTION IN NOD MICE

被引：23

作者：

FUKUDA, M ^{[1
]}

IKEGAMI, H ^{[1
]}

KAWAGUCHI, Y ^{[1
]}

SANO, T ^{[1
]}

OGIHARA, T ^{[1
]}

机构：

[1] TOKUSHIMA UNIV MED, DEPT PATHOL, TOKUSHIMA, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 209卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1590

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preventive effect of antioxidant on the islet cell destruction in NOD mice was studied. Oral administration of probucol prevented the development of cyclophosphamide induced diabetes in NOD mice (incidence of diabetes; probucol: 0/10 vs. control: 9/15) As in vitro studies, the effects of probucol on the generation of H2O2 in the content of H2O2 in islet cells co-cultured with Mo were measured. Intracellular oxidation of 2'7'-dichlorofluorescin by H2O2 was analyzed by flow cytometry. The probucol-treated Ms induced less marked increase of H2O2 in islet cells in comparison with control Ma. The generation of H2O2 by Mo in islet cells from treated mice was less marked than that in controls. These results suggest that the preventive effect of probucol may be mediated by protection of islet cells from oxidative stress induced by Mo. (C) 1995 Academic Press, Inc.

引用

页码：953 / 958

页数：6

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