A MICROASSAY FOR PROTEIN GLYCATION BASED ON THE PERIODATE METHOD

被引：40

作者：

AHMED, N

FURTH, AJ

机构：

[1] Oxford Research Unit, The Open University, Oxford, OX1 5HR England, Boars Hill

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90193-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The periodate assay for glycated protein has been adapted for use with a microplate reader. Up to 94 samples can be read in 40 s, and sensitivity has been improved so that only 2-40 nmol of protein-bound sugar are needed per well. Yields have been increased to over 90%, allowing in vitro glycation of human serum albumin to be assayed on 0.1-mg aliquots. © 1991.

引用

收藏

页码：109 / 111

页数：3

相关论文

共 12 条

[1]

BAYNES JW, 1989, MAILLARD REACTION AG, P43

[2] QUANTITATION OF GLYCOSYLATED HEMOGLOBIN - ELIMINATION OF LABILE GLYCOHEMOGLOBIN DURING SAMPLE HEMOLYSIS AT PH-5 [J].

BISSE, E ;

BERGER, W ;

FLUCKIGER, R .

DIABETES, 1982, 31 (07) :630-633

[3]

BRIGHTON MW, 1988, CLIN CHEM, V34, P2378

[4] ADVANCED PRODUCTS OF NONENZYMATIC GLYCOSYLATION AND THE PATHOGENESIS OF DIABETIC VASCULAR-DISEASE [J].

BROWNLEE, M ;

CERAMI, A ;

VLASSARA, H .

DIABETES-METABOLISM REVIEWS, 1988, 4 (05) :437-451

[5] METHODS FOR ASSAYING NONENZYMATIC GLYCOSYLATION [J].

FURTH, AJ .

ANALYTICAL BIOCHEMISTRY, 1988, 175 (02) :347-360

[6] CHEMICAL QUANTITATION OF HEMOGLOBIN GLYCOSYLATION - FLUOROMETRIC DETECTION OF FORMALDEHYDE RELEASED UPON PERIODATE-OXIDATION OF GLYCOGLOBIN [J].

GALLOP, PM ;

FLUCKIGER, R ;

HANNEKEN, A ;

MININSOHN, MM ;

GABBAY, KH .

ANALYTICAL BIOCHEMISTRY, 1981, 117 (02) :427-432

[7]

MCDONALD MJ, 1978, J BIOL CHEM, V253, P2327

[8] ROLE OF FRUCTOSE IN GLYCATION AND CROSS-LINKING OF PROTEINS [J].

MCPHERSON, JD ;

SHILTON, BH ;

WALTON, DJ .

BIOCHEMISTRY, 1988, 27 (06) :1901-1907

[9] THE COLORIMETRIC ESTIMATION OF FORMALDEHYDE BY MEANS OF THE HANTZSCH REACTION [J].

NASH, T .

BIOCHEMICAL JOURNAL, 1953, 55 (03) :416-421

[10] THE STANDARDIZATION OF THE THIOBARBITURIC ACID ASSAY FOR NON-ENZYMATIC GLUCOSYLATION OF HUMAN-SERUM ALBUMIN [J].

NEY, KA ;

COLLEY, KJ ;

PIZZO, SV .

ANALYTICAL BIOCHEMISTRY, 1981, 118 (02) :294-300