PRODUCTION OF MONOCLONAL-ANTIBODY REACTIVE WITH LEUKOTOXIN AND ITS IMMUNOHISTOCHEMICAL APPLICATION

被引：4

作者：

NAGAO, M

ZHANG, WD

YAMADA, Y

TAKATORI, T

IWASE, H

IWADATE, K

OONO, T

机构：

[1] Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo

来源：

FORENSIC SCIENCE INTERNATIONAL | 1994年 / 69卷 / 02期

关键词：

LEUKOTOXIN (9,10-EPOXY-12-OCTADECENOIC ACID); MONOCLONAL ANTIBODY; IMMUNOHISTOCHEMISTRY; MACROPHAGE;

D O I：

10.1016/0379-0738(94)90249-6

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A sensitive enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (ALM-1) was developed for the determination of leukotoxin (9,10-epoxy-12-octadecenoic acid), which was reported to exist in human burned skin and neutrophils, and was regarded as a toxic and/or defensive substance in living beings. The leukotoxin was conjugated with bovine serum albumin (BSA) by means of the mixed anhydride technique as immunogen, and BALB/c mice were immunized over 6 months. The detection limit of leukotoxin was at least as low as 5 ng in this ELISA. This antibody had a strong specificity to leukotoxin and no cross-reactivity with the other analogs tested, and was usable for an immunohistochemical application. By using the antibody, leukotoxin Was immunohistochemically observed not only in neutrophils, but also in alveolar macrophages in an oxygen-exposured rat lung.

引用

页码：131 / 138

页数：8

共 15 条

[1] AKABANE H, 1993, JPN J LEGAL MED, V47, P93
[2] Akabane H., 1991, JPN J CLIN CHEM, V20, P203
[3] ERLANGER BF, 1957, J BIOL CHEM, V228, P713
[4] NEUTROPHILS BIOSYNTHESIZE LEUKOTOXIN, 9-EPOXY-12-OCTADECENOATE, 10-EPOXY-12-OCTADECENOATE
HAYAKAWA, M
SUGIYAMA, S
TAKAMURA, T
YOKOO, K
IWATA, M
SUZUKI, K
TAKI, F
TAKAHASHI, S
OZAWA, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 137 (01) : 424 - 430
[5] Hayakawa Y., 1986, J CLIN BIOCHEM NUTR, V1, P121
[6] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY
KOHLER, G
MILSTEIN, C
[J]. NATURE, 1975, 256 (5517) : 495 - 497
[7] NAGAO M, 1990, TOXICOLOGY, V63, P167, DOI 10.1016/0300-483X(90)90040-N
[8] NAGAO M, 1989, CLIN CHEM ENZYM COMM, V1, P221
[9] OUCHERLONY O, 1949, ACTA PATHOL MIC SC, V26, P51
[10] BIOSYNTHESIS OF LEUKOTOXIN, 9, 10-EPOXY-12 OCTADECENOATE, BY LEUKOCYTES IN LUNG LAVAGES OF RAT AFTER EXPOSURE TO HYPEROXIA
OZAWA, T
HAYAKAWA, M
TAKAMURA, T
SUGIYAMA, S
SUZUKI, K
IWATA, M
TAKI, F
TOMITA, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 134 (03) : 1071 - 1078

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