PREPARATION AND PURIFICATION OF BETA-AMYLOID(1-43) VIA SOLUBLE, AMIDE BACKBONE PROTECTED INTERMEDIATES

被引：49

作者：

QUIBELL, M ^{[1
]}

TURNELL, WG ^{[1
]}

JOHNSON, T ^{[1
]}

机构：

[1] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1994年 / 59卷 / 07期

关键词：

D O I：

10.1021/jo00086a025

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report a new synthetic strategy for the preparation of the amyloidogenic protein fragment beta A4, through an easily purified (and characterized) intermediate of high aqueous solubility. This novel intermediate consists of beta A4(1-43) with backbone amide protection, provided by the N-(2-hydroxy-4-methoxybenzyl) group, on residues Phe(20) and Gly(25,29,33,38). The new strategy enabled derivatized beta A4(1-43) to be isolated and purified by standard reverse-phase HPLC procedures and characterized by electrospray mass spectrometry (ESMS).

引用

页码：1745 / 1750

页数：6

共 25 条

[1]

Atherton E., 1985, PEPTIDES STRUCTURE F, P415

[2]

Atherton E., 1989, SOLID PHASE PEPTIDE, P1

[3] SOLUTION STRUCTURES OF BETA PEPTIDE AND ITS CONSTITUENT FRAGMENTS - RELATION TO AMYLOID DEPOSITION [J].