LARGE ACTIVATION OF SERINE PROTEASES BY PRETREATMENT WITH CROWN-ETHERS

被引：56

作者：

BROOS, J ^{[1
]}

SAKODINSKAYA, IK ^{[1
]}

ENGBERSEN, JFJ ^{[1
]}

VERBOOM, W ^{[1
]}

REINHOUDT, DN ^{[1
]}

机构：

[1] UNIV TWENTE, ORGAN CHEM LAB, 7500 AE ENSCHEDE, NETHERLANDS

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1995年 / 02期

关键词：

D O I：

10.1039/c39950000255

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Pretreatment of serine proteases by lyophilisation in the presence of crown ethers leads to large enhancements of enzyme activity in organic solvents.

引用

页码：255 / 256

页数：2

共 22 条

[1] ENGINEERING ENZYMES FOR NONAQUEOUS SOLVENTS [J].

ARNOLD, FH .

TRENDS IN BIOTECHNOLOGY, 1990, 8 (09) :244-249

[2]

Boyer PD, 1971, ENZYMES, VIII

[3]

BROOS J, 1991, RECL TRAV CHIM PAY B, V110, P222

[4]

BROOS J, IN PRESS

[5]

BROOS J, 1994, THESIS U TWENTE NETH

[6] DRAMATIC ENHANCEMENT OF ENZYMATIC-ACTIVITY IN ORGANIC-SOLVENTS BY LYOPROTECTANTS [J].

DABULIS, K ;

KLIBANOV, AM .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (05) :566-571

[7]

DEJONG F, 1976, TETRAHEDRON LETT, P1371

[8] DESIGNING ENZYMES FOR USE IN ORGANIC-SOLVENTS [J].

DORDICK, JS .

BIOTECHNOLOGY PROGRESS, 1992, 8 (04) :259-267

[9]

GOLOVKOVA LP, 1985, THEOR EXP CHEM, V2, P248

[10]

HALLING PJ, 1992, BIOTECHNOL TECH, V6, P271

← 1 2 3 →