HORMONAL-CONTROL OF APOLYSIS IN BARNACLE MANTLE TISSUE EPIDERMIS, INVITRO

被引:32
作者
FREEMAN, JA
COSTLOW, JD
机构
[1] Duke University Marine Laboratory, Beaufort, North Carolina
来源
JOURNAL OF EXPERIMENTAL ZOOLOGY | 1979年 / 210卷 / 02期
关键词
D O I
10.1002/jez.1402100217
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Ecdysterone (5–20 μg/ml) stimulated apolysis in inner mantle tissue explants of the barnacle, Balanus amphitrite, maintained under in vitro conditions. Apolysis was noticeable after 12 to 15 hours while complete apolysis was reached by 24 hours. Enlargement of the epidermal cells was also observed in ecdysterone‐stimulated tissues. Ecdysone (10–20 μg/ml) was equally as effective as ecdysterone in stimulating apolysis. In some tissues, which had been cultured with 20 μg/ml ecdysterone for 24 hours, new cuticle formation had occurred following apolysis. The initiation of apolysis by ecdysterone was dependent on RNA and protein synthesis, as indicated by the inhibition of ecdysterone‐promoted apolysis by actinomycin D and cycloheximide. Aqueous extracts of shrimp eyestalks (MIH) (10 es eq/ml), either as a crude extract, or as a low molecular weight fraction from a Sephadex G‐25 column, were active in repressing the apolysis‐stimulating action of ecdysterone. The MIH activity could be overridden by increased levels of ecdysterone. Protease treatment of the MIH from crude and partially purified fractions abolished the inhibitory activity. Molt‐inhibiting hormone did not bind 3H‐ecdysterone. The results demonstrate that the early premolt integumental activities are under the control of ecdysterone and molt‐inhibiting hormone. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company
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页码:333 / 345
页数:13
相关论文
共 39 条
[1]   TIME STUDIES OF ECDYSONE-ACTION ON INVITRO APOLYSIS OF CHILO-SUPPRESSALIS INTEGUMENT [J].
AGUI, N .
JOURNAL OF INSECT PHYSIOLOGY, 1977, 23 (07) :837-842
[2]   PHOTOPERIOD ENDOCRINOLOGY AND CRUSTACEAN MOLT CYCLE [J].
AIKEN, DE .
SCIENCE, 1969, 164 (3876) :149-+
[3]  
ANDRIEUX N, 1976, CR ACAD SCI D NAT, V283, P1429
[4]   DETECTION AND IDENTIFICATION OF MOLTING HORMONE (ECDYSONES) IN BARNACLE BALANUS-BALANOIDES [J].
BEBBINGTON, PM ;
MORGAN, ED .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1977, 56 (01) :77-79
[5]  
BLISS DE, 1974, NEUROSECRETION FINAL, P104
[6]   QUALITATIVE AND QUANTITATIVE-DETERMINATIONS OF ECDYSONES IN TISSUES OF CRAB, PACHYGRAPSUS-CRASSIPES, FOLLOWING MOLT INDUCTION [J].
CHANG, ES ;
SAGE, BA ;
OCONNOR, JD .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1976, 30 (01) :21-33
[7]   SECRETION OF ALPHA-ECDYSONE BY CRAB Y-ORGANS INVITRO [J].
CHANG, ES ;
OCONNOR, JD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :615-618
[8]  
CHEUNG PJ, 1974, AM ZOOL, V14
[9]   INTERMOLT CYCLE OF CIRRIPEDS - CRITERIA FOR ITS STAGES AND ITS DURATION IN BALANUS-AMPHITRITE [J].
DAVIS, CW ;
FYHN, UEH ;
FYHN, HJ .
BIOLOGICAL BULLETIN, 1973, 145 (02) :310-322
[10]  
DAVIS CW, 1974, J COMP PHYSIOL, V93, P85