THERMODYNAMIC STUDIES ON THE INTERACTION BETWEEN SODIUM NORMAL-DODECYL SULFATE AND HISTONE H2A

The thermodynamic parameters of interaction between histone H2A and sodium n-dodecyl sulphate (SDS) in aqueous solutions of pH 3.2, 6.4 and 10, measured over a wide range of SDS concentration by equilibrium dialysis at 27-degrees-C and 37-degrees-C, are discussed. The data are used to determine the free energy from the Wyman binding potential theoretical model, and the enthalpy of interaction from the temperature dependence of the equilibrium constants using the van't Hoff relation. The data obtained for H2A are also compared with corresponding data for histone H2B.