PROTEASOME SUBUNITS ENCODED IN THE MHC ARE NOT GENERALLY REQUIRED FOR THE PROCESSING OF PEPTIDES BOUND BY MHC CLASS-I MOLECULES

ANTIGEN processing provides major histocompatibility complex (MHC) class I molecules with short peptides, which they selectively bind and present to cytotoxic T lymphocytes1-4. The proteolytic system generating these peptides in the cytosol is unidentified, but their delivery into the endoplasmic reticulum is mediated by the TAP1-TAP2 transporter encoded in the MHC class II region5-9. Closely linked to TAP1 and TAP2 are genes for the LMP2 and LMP7 proteins10, which resemble components of proteasomes11-13, proteolytic complexes known to degrade cytosolic proteins14. This association has led to the common assumption that proteasomes function in this immunological pathway (discussed in ref. 15). We now show that the expression of stably assembled class I molecules and apparently normal peptide processing can be completely restored in the absence of LMP2 and LMP7 in the human lymphoblastoid cell line mutant 721.174 (refs 16, 17). The identity of LMP7 is directly confirmed by reconstitution of a proteasomal subunit after gene transfer. These results therefore dispute the hypothetical involvement of proteasomes in antigen processing, although a more subtle effect of LMP2 and LMP7 cannot be ruled out.