SEX DETERMINATION OF BOVINE EMBRYOS BY RESTRICTION FRAGMENT POLYMORPHISMS OF PCR AMPLIFIED ZFX ZFY LOCI

被引：25

作者：

POLLEVICK, GD

GIAMBIAGI, S

MANCARDI, S

DELUCA, L

BURRONE, O

FRASCH, ACC

UGALDE, RA

机构：

[1] INST INVEST BIOQUIM FUNDACION CAMPOMAR,ANTONIO MACHADO 151,RA-1405 BUENOS AIRES,ARGENTINA

[2] TECHNOL GENET,BUENOS AIRES,ARGENTINA

[3] INT CTR GENET ENGN & BIOTECHNOL,I-34012 TRIESTE,ITALY

[4] CABANA SORPRESA,TORQUINST,ARGENTINA

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 07期

关键词：

D O I：

10.1038/nbt0792-805

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have obtained the DNA sequence of a region from the bovine ZFY and ZFX loci and used it to design nested primers that, together with a previously described PstI polymorphism, were applied in a sensitive PCR assay to determine the sex of embryo biopsies.

引用

收藏

页码：805 / 807

页数：3

相关论文

共 11 条

[1] AMPLIFICATION OF THE ZFY AND ZFX GENES FOR SEX IDENTIFICATION IN HUMANS, CATTLE, SHEEP AND GOATS [J].

AASEN, E ;

MEDRANO, JF .

BIO-TECHNOLOGY, 1990, 8 (12) :1279-1281

[2] THE USE OF MALE-SPECIFIC CHROMOSOMAL DNA FRAGMENTS TO DETERMINE THE SEX OF BOVINE PREIMPLANTATION EMBRYOS [J].

BONDIOLI, KR ;

ELLIS, SB ;

PRYOR, JH ;

WILLIAMS, MW ;

HARPOLD, MM .

THERIOGENOLOGY, 1989, 31 (01) :95-104

[3] ENZYMATIC AMPLIFICATION OF A Y-CHROMOSOME REPEAT IN A SINGLE BLASTOMERE ALLOWS IDENTIFICATION OF THE SEX OF PREIMPLANTATION MOUSE EMBRYOS [J].

BRADBURY, MW ;

ISOLA, LM ;

GORDON, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4053-4057

[4] RECENT ADVANCES IN THE POLYMERASE CHAIN-REACTION [J].

ERLICH, HA ;

GELFAND, D ;

SNINSKY, JJ .

SCIENCE, 1991, 252 (5013) :1643-1651

[5] PREGNANCIES FROM BIOPSIED HUMAN PREIMPLANTATION EMBRYOS SEXED BY Y-SPECIFIC DNA AMPLIFICATION [J].

HANDYSIDE, AH ;

KONTOGIANNI, EH ;

HARDY, K ;

WINSTON, RML .

NATURE, 1990, 344 (6268) :768-770

[6]

HERR C M, 1990, Theriogenology, V33, P245, DOI 10.1016/0093-691X(90)90669-K

[7]

HERR C M, 1990, Theriogenology, V33, P247, DOI 10.1016/0093-691X(90)90671-F

[8]

Maniatis T, 1989, MOL CLONING

[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10] SHEDDING LIGHT ON PCR CONTAMINATION [J].

SARKAR, G ;

SOMMER, SS .

NATURE, 1990, 343 (6253) :27-27