FUNCTIONAL-SIGNIFICANCE OF CONSERVED AMINO-ACID-RESIDUES

被引：34

作者：

POTEETE, AR

RENNELL, D

BOUVIER, SE

机构：

[1] Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester, Massachusetts

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1992年 / 13卷 / 01期

关键词：

BACTERIOPHAGE LYSOZYME; MUTATION; PROTEIN EVOLUTION;

D O I：

10.1002/prot.340130104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A systematic study of single amino acid substitutions in bacteriophage T4 lysozyme permitted a test of the concept that conserved amino acid residues are more functionally important than nonconserved residues. Substitutions of amino acid residues that are conserved among five bacteriophage-encoded lysozymes were found to lead more frequently to loss of function than substitutions of nonconserved residues. Of 163 residues tested, only 74 (45%) are sensitive to at least one substitution; however, all 14 residues that are fully conserved are sensitive to substitutions.

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页码：38 / 40

页数：3

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