POSSIBLE REGULATION OF CFTR-CHLORIDE CHANNELS BY MEMBRANE-BOUND PHOSPHATASES IN PANCREATIC DUCT CELLS

被引：56

作者：

BECQ, F

FANJUL, M

MERTEN, M

FIGARELLA, C

HOLLANDE, E

GOLA, M

机构：

[1] CNRS,NEUROBIOL LAB,LNB4,31 CHEMIN JOSEPH AIGUIER,F-13402 MARSEILLE 9,FRANCE

[2] UNIV TOULOUSE 3,BIOL CELLULAIRE LAB,F-31400 TOULOUSE,FRANCE

[3] GRP RECH GLANDES EXOCRINES,F-13009 MARSEILLE,FRANCE

来源：

FEBS LETTERS | 1993年 / 327卷 / 03期

关键词：

CFTR-CHLORIDE CHANNEL; PHOSPHATASE; PANCREAS;

D O I：

10.1016/0014-5793(93)81016-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied CFTR-Cl- channels in non-CF CAPAN-1 and in CFTR-transfected CFPAC-PLJ-CFTR-6 epithelial cells from human pancreas. Theophylline and IBMX induced the opening of cell-attached CFTR-Cl- channels. Theophylline, IBMX and the alkaline phosphatase (AP) inhibitor levamisole enhanced the activity of excised channels and reduced by 70-75% the apical membrane-associated APs activity. Okadaic acid had no effect on APs and channel activities. A polyclonal anti-alkaline phosphatase antibody (which detected apical APs) reduced APs activity and activated quiescent excised chloride channels. These results suggest that CFTR channels may be regulated by membrane-bound phosphatases.

引用

收藏

页码：337 / 342

页数：6

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