INHIBITION BY DEXAMETHASONE OF RETINOIC ACID-INDUCED ENHANCEMENT OF LEUKOTRIENE C-4 SYNTHESIS IN RAT BASOPHILIC LEUKEMIA-1 CELLS

被引:19
作者
HAMASAKI, Y [1 ]
ABE, M [1 ]
MATSUMOTO, S [1 ]
ICHIMARU, T [1 ]
KOBAYASHI, I [1 ]
TANAKA, E [1 ]
MATSUO, M [1 ]
HARA, N [1 ]
MIYAZAKI, S [1 ]
机构
[1] KYUSHU UNIV,FAC MED,DIS CHEST RES INST,FUKUOKA,JAPAN
关键词
D O I
10.1165/ajrcmb.11.1.8018338
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated inhibitory actions of dexamethasone (DEX) on retinoic acid (RA)-induced enhancement of leukotriene C4 (LTC4) synthesis in rat basophilic leukemia-1 (RBL-1) cells. Cultured cells were preincubated with RA for 16 h with or without DEX, and generation of LTC4 was measured by high performance liquid chromatography in cell-free and intact cell systems. RA (0.1 mug/ml) significantly potentiated calcium ionophore-stimulated production of LTC4 synthesis. DEX inhibited the RA-induced enhancement of LTC4 synthesis by up to approximately 95 % in intact cells when stimulated with calcium ionophore. RA-induced LTC4 synthase activity, which was determined by enzyme assay, was also inhibited by DEX by 65 % in a cell-free system. This discrepancy of inhibition between the intact and cell-free systems was due to a partial inhibition of phospholipase A2 activity by DEX in the intact cells. These results indicate that the production of LTC4 is predominantly regulated at a level of LTC4 synthase. The induction of new LTC4 Synthase activity by RA and inhibition of the RA-induced activity by DEX are important regulatory mechanisms of LTC4 synthesis.
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页码:49 / 56
页数:8
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共 52 条
  • [1] CHARACTERIZATION OF LEUKOTRIENE-C4 SYNTHETASE IN MOUSE PERITONEAL-EXUDATE CELLS
    ABE, M
    HUGLI, TE
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 959 (03) : 386 - 398
  • [2] ENHANCED LEUKOTRIENE-C4 SYNTHASE ACTIVITY IN THIOGLYCOLLATE-ELICITED PERITONEAL-MACROPHAGES
    ABE, M
    HARA, N
    MURANISHI, H
    IKEDA, T
    NAGATA, N
    SHIGEMATSU, N
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (03) : 1344 - 1352
  • [3] BORGEAT P, 1979, J BIOL CHEM, V254, P2643
  • [4] CASEY ML, 1988, J BIOL CHEM, V263, P7846
  • [5] A NOVEL ARACHIDONIC ACID-SELECTIVE CYTOSOLIC PLA2 CONTAINS A CA2+-DEPENDENT TRANSLOCATION DOMAIN WITH HOMOLOGY TO PKC AND GAP
    CLARK, JD
    LIN, LL
    KRIZ, RW
    RAMESHA, CS
    SULTZMAN, LA
    LIN, AY
    MILONA, N
    KNOPF, JL
    [J]. CELL, 1991, 65 (06) : 1043 - 1051
  • [6] PURIFICATION OF A 110-KILODALTON CYTOSOLIC PHOSPHOLIPASE-A2 FROM THE HUMAN MONOCYTIC CELL-LINE U937
    CLARK, JD
    MILONA, N
    KNOPF, JL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) : 7708 - 7712
  • [7] CHARACTERIZATION AND PARTIAL-PURIFICATION OF RENOCORTINS-2 POLYPEPTIDES FORMED IN RENAL-CELLS CAUSING THE ANTI-PHOSPHOLIPASE-LIKE ACTION OF GLUCOCORTICOIDS
    CLOIX, JF
    COLARD, O
    ROTHHUT, B
    RUSSOMARIE, F
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1983, 79 (01) : 313 - 321
  • [8] LEUKOTRIENE-B - TOTAL SYNTHESIS AND ASSIGNMENT OF STEREOCHEMISTRY
    COREY, EJ
    MARFAT, A
    GOTO, G
    BRION, F
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (27) : 7984 - 7985
  • [9] PEPTIDE LEUKOTRIENE RELEASE AFTER ANTIGEN CHALLENGE IN PATIENTS SENSITIVE TO RAGWEED
    CRETICOS, PS
    PETERS, SP
    ADKINSON, NF
    NACLERIO, RM
    HAYES, EC
    NORMAN, PS
    LICHTENSTEIN, LM
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1984, 310 (25) : 1626 - 1630
  • [10] ALLERGEN CHALLENGE OF LUNG-TISSUE FROM ASTHMATICS ELICITS BRONCHIAL CONTRACTION THAT CORRELATES WITH THE RELEASE OF LEUKOTRIENE-C4, LEUKOTRIENE-D4, AND LEUKOTRIENE-E4
    DAHLEN, SE
    HANSSON, G
    HEDQVIST, P
    BJORCK, T
    GRANSTROM, E
    DAHLEN, B
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06): : 1712 - 1716