FRACTIONATION OF MAMMALIAN DNA BY ZONAL CENTRIFUGATION

被引:4
作者
HALSALL, HB
SCHUMAKER, VN
机构
[1] Department of Chemistry, Molecular Biology Institute, University of California, Los Angeles, CA
关键词
D O I
10.1038/221772a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
COMMONLY used isolation procedures for mammalian DNA invariably give a product possessing a wide distribution of sedimentation coefficients1,2. We are interested in reproducibly obtaining finely cut fractions of such heterogeneous samples of DNA in order to study the production of such distributions, with the problems of shear degradation reduced to a minimum. Schumaker, Richards and Freer3 described the fractionation of calf thymus DNA. Their technique using rate zonal centrifugation in tubes, however, has inherent disadvantages. Tubes are low in capacity, zones may be subject to convection or anomalous sedimentation4-6, and problems of shear degradation may arise during fractionation7. © 1969 Nature Publishing Group.
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页码:772 / +
页数:1
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