PREPARATIVE FRACTIONATION OF NUCLEIC-ACIDS BY AGAROSE-GEL ELECTROPHORESIS

被引：12

作者：

BOSTIAN, KA ^{[1
]}

LEE, RC ^{[1
]}

HALVORSON, HO ^{[1
]}

机构：

[1] BRANDEIS UNIV, DEPT BIOL, WALTHAM, MA 02154 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90201-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Agarose gel electrophoresis offers a versatile means to fractionate nucleic acids varying in size over considerably different molecular weight ranges. Surface cohesion properties of agarose gels and sample loading problems have hampered the use of such gels in largediameter, preparative-scale tube gel electrophoresis. We report here a procedure that makes routine and reproducible the construction, sample loading, and running of preparative agarose electrophoretic gels. Data are presented on the fractionation of yeast nucleic acids. © 1979.

引用

页码：174 / 182

页数：9

共 35 条

[1] AVIN H, 1972, P NATL ACAD SCI USA, V69, P1408
[2] METHYLMERCURY AS A REVERSIBLE DENATURING AGENT FOR AGAROSE-GEL ELECTROPHORESIS
BAILEY, JM
DAVIDSON, N
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) : 75 - 85
[3] ELECTROPHORETIC SEPARATION OF VIRAL NUCLEIC ACIDS ON POLYACRYLAMIDE GELS
BISHOP, DHL
CLAYBROO.JR
SPIEGELM.S
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1967, 26 (03) : 373 - &
[4] CONFORMATION INDEPENDENT MOLECULAR WEIGHT DETERMINATIONS OF RNA BY GEL ELECTROPHORESIS
BOEDTKER, H
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 240 (03) : 448 - +
[5] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS
BONNER, WM
LASKEY, RA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01): : 83 - 88
[6] BOSTIAN KA, UNPUBLISHED
[7] SIMPLE METHOD FOR ISOLATION OF UNDEGRADED GIANT RNA MOLECULES
CASE, ST
DANEHOLT, B
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 74 (01) : 198 - 206
[8] ANALYTICAL STUDIES ON NUCLEAR RIBONUCLEIC ACID USING POLYACRYLAMIDE GEL ELECTROPHORESIS
DINGMAN, CW
PEACOCK, AC
[J]. BIOCHEMISTRY, 1968, 7 (02) : 659 - &
[9] DINGMAN CW, 1971, PROCEDURES NUCLEIC A, V2, P623
[10] SEPARATION OF SINGLE-STRANDED RIBONUCLEIC-ACIDS BY ACRYLAMIDE-AGAROSE-UREA GEL-ELECTROPHORESIS
FLOYD, RW
STONE, MP
JOKLIK, WK
[J]. ANALYTICAL BIOCHEMISTRY, 1974, 59 (02) : 599 - 609

← 1 2 3 4 →