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SEPARATION OF MANNOSYLRETINYLPHOSPHATE FROM DOLICHYLMANNOSYLPHOSPHATE BY CHROMATOGRAPHY ON COLUMNS OF DEAE-SEPHACEL

被引：8
作者：
SASAK, W
SILVERMANJONES, CS
DE LUCA, LM
机构：
[1] National Institutes of Health, Bethesda
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 02期
关键词：
D O I：
10.1016/0003-2697(79)90075-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[14C]mannose in the absence or presence of exogenous retinylphosphate. © 1979.
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页码：298 / 301
页数：4
相关论文
共 13 条
[1]   INVIVO INCORPORATION OF MANNOSE, RETINOL AND MEVALONIC ACID INTO PHOSPHOLIPIDS OF HAMSTER LIVER [J].
BARR, RM ;
DE LUCA, LM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 60 (01) :355-363
[2]   GLYCOSYL TRANSFER FROM NUCLEOTIDE SUGARS TO C85- AND C55-POLYPRENYL AND RETINYL PHOSPHATES BY MICROSOMAL SUBFRACTIONS AND GOLGI MEMBRANES OF RAT-LIVER [J].
BERGMAN, A ;
MANKOWSKI, T ;
CHOJNACKI, T ;
DE LUCA, LM ;
PETERSON, E ;
DALLNER, G .
BIOCHEMICAL JOURNAL, 1978, 172 (01) :123-127
[3]  
de Luca L.M., 1977, Vitamins and Hormones, V35, P1
[4]  
DE LUCA LM, 1977, J BIOL CHEM, V252, P2575
[5]  
FROTCOUTAZ JP, 1976, J LIPID RES, V17, P220
[6]  
HASSELL JR, 1978, J BIOL CHEM, V253, P1627
[7]  
LITTLEFIELD JW, 1957, J BIOL CHEM, V224, P13
[8]   INVOLVEMENT OF ENDOGENOUS DOLICHOL IN FORMATION OF LIPID-LINKED PRECURSORS OF GLYCOPROTEIN IN RAT-LIVER [J].
MARTIN, HG ;
THORNE, KJI .
BIOCHEMICAL JOURNAL, 1974, 138 (02) :281-289
[9]  
MASUSHIGE S, 1978, J LIPID RES, V19, P619
[10]   STUDIES ON URIDINE-DIPHOSPHATE-GLUCOSE [J].
PALADINI, AC ;
LELOIR, LF .
BIOCHEMICAL JOURNAL, 1952, 51 (03) :426-430
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