A SEMI-MICRO QUANTITATIVE ASSAY FOR DETERMINATION OF CHITINOLYTIC ACTIVITY IN MICROORGANISMS

被引：4

作者：

EVRALL, CC ^{[1
]}

ATTWELL, RW ^{[1
]}

SMITH, CA ^{[1
]}

机构：

[1] MANCHESTER POLYTECH,DEPT BIOL SCI,CHESTER ST,MANCHESTER M1 5GD,LANCS,ENGLAND

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1990年 / 12卷 / 3-4期

关键词：

CHITINASE; CHITIN-AZURE; CHITINOLYTIC ACTIVITY; QUANTITATIVE ASSAY;

D O I：

10.1016/0167-7012(90)90029-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A semi-micro quantitative assay for microbial chitinolytic activity has been developed. The amount of dye released from a chitin-azure substrate by chitinase producing organisms was determined densitometrically and used as a measure of chitinolytic activity. This activity could be expressed as units of chitinase.mg-1 of inoculum protein, after calibration of the system. A linear relationship was found between dye release and inoculum protein content in constitutive and induced microoganisms. The assay described offers advantages over existing techniques. It is simple, quick and suitable for use in screening programmes or in studies on enzyme kinetics.

引用

页码：183 / 187

页数：5

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