COMPARISON OF THE EARLY PRODUCTION OF EXTRACELLULAR-MATRIX ON DENSE HYDROXYAPATITE AND HYDROXYAPATITE-COATED TITANIUM IN CELL AND ORGAN-CULTURE

被引:32
作者
COURTENEYHARRIS, RG
KAYSER, MV
DOWNES, S
机构
[1] ROYAL NATL ORTHOPAED HOSP TRUST,IRC BIOMED MAT,STANMORE HA7 4LP,MIDDX,ENGLAND
[2] ROYAL NATL ORTHOPAED HOSP TRUST,INST ORTHOPAED,STANMORE HA7 4LP,MIDDX,ENGLAND
关键词
HYDROXYAPATITES; CELL CULTURE; ORGAN CULTURE; EXTRACELLULAR MATRIX;
D O I
10.1016/0142-9612(95)98823-W
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In order to investigate the earliest changes at the bone-hydroxyapatite (HA) interface, two materials, dense HA and HA-coated titanium alloy, were investigated for their ability to stimulate production of extracellular matrix both in cell and organ culture. Osteoblast-like cells were obtained from rabbit calvariae after preliminary culture for 3 d and seeded out onto the two materials. When these cells were grown in culture on the dense HA no matrix production was seen with the scanning electron microscope after 4 d, but there did appear to be matrix produced on the rougher surface of the HA coating. In contrast, extracellular matrix production was greatly increased in organ culture where the material was placed on a section of bone, particularly on the HA coating, where it formed an organized layer of collagen within 4 d. These results showed that extracellular matrix production by both osteoblastic cells and in calvarial culture was greater on the HA coatings than dense HA. In addition, organ culture allows the investigation of the earliest changes in the bone-HA interface without having to undertake in vivo implantation studies of such short duration.
引用
收藏
页码:489 / 495
页数:7
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