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CYTOPLASMIC DNAS OF CULTURED HUMAN CELLS

被引：12
作者：
KOCH, J
机构：
[1] Department of Nutritional Sciences, University of California, Berkeley
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00773.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In cultured human cells (Chang, liver) labeled thymidine is incorporated during short labeling periods into a cytoplasmic DNA, presumably located in the mitochondria, with a buoyant density of 1.688 g/ml (nuclear DNA main band 1.700 g/ml). This DNA is apparently metabolically labile and becomes completely delabeled after a chasing period of approximately three cell generations. During such a chase period an increasing amount of radioactive label appears in closed circular duplex DNA (buoyant density 1.706 g/ml). Pulse ([Me‐3H]thymidine)–chase–pulse ([2‐14C]‐thymidine) experiments suggest that the “light cytoplasmic” DNA is a precursor of the closed circular duplex DNA. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：296 / &
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