A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE

被引：1092

作者：

BRADY, L

BRZOZOWSKI, AM

DEREWENDA, ZS

DODSON, E

DODSON, G

TOLLEY, S

TURKENBURG, JP

CHRISTIANSEN, L

HUGEJENSEN, B

NORSKOV, L

THIM, L

MENGE, U

机构：

[1] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND

[2] NOVO NORDISK A-S,DK-2880 BAGSVAERD,DENMARK

[3] GBF,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY

来源：

NATURE | 1990年 / 343卷 / 6260期

关键词：

D O I：

10.1038/343767a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

True Upases attack triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop. © 1990 Nature Publishing Group.

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页码：767 / 770

页数：4

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