MEASUREMENT OF ALKALINE-PHOSPHATASE OF INTESTINAL ORIGIN IN PLASMA BY P-BROMOTETRAMISOLE INHIBITION

被引:9
作者
KUWANA, T [1 ]
ROSALKI, SB [1 ]
机构
[1] ROYAL FREE HOSP,DEPT CHEM PATHOL & HUMAN METAB,LONDON NW3 2QG,ENGLAND
关键词
D O I
10.1136/jcp.44.3.236
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.
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页码:236 / 237
页数:2
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