UNUSUAL SOLVENT EFFECT ON PROTEASE ACTIVITY AND EFFECTIVE OPTICAL RESOLUTION OF AMINO-ACIDS BY HYDROLYTIC REACTIONS IN ORGANIC-SOLVENTS

被引：35

作者：

KISE, H

TOMIUCHI, Y

机构：

[1] Institute of Materials Science, University of Tsukuba, Tsukuba

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 05期

关键词：

D O I：

10.1007/BF01027675

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The catalytic activities of alpha-chymotrypsin, subtilisin Carlsberg, and subtilisin BPN' for hydrolysis of amino acid esters in acetonitrile-water were unusually dependent on the solvent composition. The products obtained as precipitates in high concentrations of acetonitrile were L-amino acids of high optical purities, and effective optical resolution of amino acids was achieved.

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页码：317 / 322

页数：6

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共 11 条

[1] GENERAL-ASPECTS AND OPTIMIZATION OF ENANTIOSELECTIVE BIOCATALYSIS IN ORGANIC-SOLVENTS - THE USE OF LIPASES [J].

CHEN, CS ;

SIH, CJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06) :695-707

[2] ENZYMATIC CATALYSIS IN MONOPHASIC ORGANIC-SOLVENTS [J].

DORDICK, JS .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (04) :194-211

[3] STABILIZATION EFFECTS ON IMMOBILIZED YEAST - EFFECT OF GEL COMPOSITION ON TOLERANCE TO WATER MISCIBLE SOLVENTS [J].

DROR, Y ;

COHEN, O ;

FREEMAN, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (05) :273-279

[4] SYNTHESIS OF PEPTIDE-BONDS BY PROTEINASES - ADDITION OF ORGANIC COSOLVENTS SHIFTS PEPTIDE-BOND EQUILIBRIA TOWARD SYNTHESIS [J].

HOMANDBERG, GA ;

MATTIS, JA ;

LASKOWSKI, M .

BIOCHEMISTRY, 1978, 17 (24) :5220-5227

[5] BASIC PRINCIPLES OF PROTEASE-CATALYZED PEPTIDE-BOND FORMATION [J].

JAKUBKE, HD ;

KUHL, P ;

KONNECKE, A .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1985, 24 (02) :85-93

[6] MECHANISM AND YIELDS IN ENZYME CATALYZED EQUILIBRIUM AND KINETICALLY CONTROLLED SYNTHESIS OF BETA-LACTAM ANTIBIOTICS, PEPTIDES AND OTHER CONDENSATION PRODUCTS [J].

KASCHE, V .

ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (01) :4-16

[7] ENGINEERING BIOCATALYTIC SYSTEMS IN ORGANIC MEDIA WITH LOW WATER-CONTENT [J].

KHMELNITSKY, YL ;

LEVASHOV, AV ;

KLYACHKO, NL ;

MARTINEK, K .

ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (12) :710-724

[8] PROTEASE-CATALYZED SYNTHETIC REACTIONS AND IMMOBILIZATION-ACTIVATION OF THE ENZYMES IN HYDROPHILIC ORGANIC-SOLVENTS [J].

KISE, H ;

HAYAKAWA, A ;

NORITOMI, H .

JOURNAL OF BIOTECHNOLOGY, 1990, 14 (3-4) :239-254

[9] ESTER AND PEPTIDE-SYNTHESIS BY PROTEASES IN ORGANIC-SOLVENTS [J].

KISE, H .

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN, 1991, 49 (01) :42-51

[10] ASYMMETRIC TRANSFORMATIONS CATALYZED BY ENZYMES IN ORGANIC-SOLVENTS [J].

KLIBANOV, AM .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (04) :114-120