VCAM-1 IS A RECEPTOR FOR ENCEPHALOMYOCARDITIS VIRUS ON MURINE VASCULAR ENDOTHELIAL-CELLS

Murine VCAM-1 has been identified as a receptor for the D variant of encephalomyocarditis (EMC-D) virus on vascular endothelial cells from the heart. Monoclonal antibodies to VCAM-1 inhibited infection and lysis of endothelial cells with EMC-D virus. CHO cells transfected with the VCAM-1 gene were susceptible to ER IC-D virus lysis, while control CHO cells transfected with the ELAM-1 gene were resistant. Similarly, S-35-labeled EMC-D virus bound to CHO-VCAM cells, and binding was inhibited with anti-VCAM-1 antibodies. Little or no radiolabeled virus bound to CHO-ELAM-1 cells.