A CIRCULAR TRANS-ACTING HEPATITIS-DELTA VIRUS RIBOZYME

被引：59

作者：

PUTTARAJU, M ^{[1
]}

PERROTTA, AT ^{[1
]}

BEEN, MD ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 18期

关键词：

D O I：

10.1093/nar/21.18.4253

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A circular trans-acting ribozyme designed to adopt the motif of the hepatitis delta virus (HDV) trans-acting ribozyme was produced. The circular form was generated in vitro by splicing a modified group I intron precursor RNA in which the relative order of the 5' and 3' splice sites, flanking the single HDV-like ribozyme sequence-containing exon, is reversed. Trans-cleavage activity of the circular HDV-like ribozyme was comparable to linear permutations of HDV ribozymes containing the same core sequence, and was shown not to be due to linear contaminants in the circular ribozyme preparation. In nuclear and cytoplasmic extracts from HeLa cells, the circular ribozyme had enhanced resistance to nuclease degradation relative to a linear form of the ribozyme, suggesting that circularization may be a viable alternative to chemical modification as a means of stabilizing ribozymes against nuclease degradation.

引用

页码：4253 / 4258

页数：6

共 47 条

[1]

BARRELL BG, 1971, PROCEDURES NUCLEIC A, V2, P751

[2] SECONDARY STRUCTURE OF THE SELF-CLEAVING RNA OF HEPATITIS DELTA-VIRUS - APPLICATIONS TO CATALYTIC RNA DESIGN [J].