ITACONATE BIOSYNTHESIS IN ASPERGILLUS-TERREUS

被引：87

作者：

BONNARME, P

GILLET, B

SEPULCHRE, AM

ROLE, C

BELOEIL, JC

DUCROCQ, C

机构：

[1] CNRS,INST CHIM SUBST NAT,F-91198 GIF SUR YVETTE,FRANCE

[2] TEXEL,ZA BUXIERES,F-86220 DANGE ST ROMAIN,FRANCE

[3] RHONE POULENC CHIM,USINE MELLE,F-79500 MELLE,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 12期

关键词：

D O I：

10.1128/jb.177.12.3573-3578.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Itaconate biosynthesis was studied in intact cells of high-yield (RC4') and low-yield (CM85J) strains of the fungus Aspergillus terreus by methods (tracers, nuclear magnetic resonance spectroscopy, and mass spectroscopy) that did not interfere with metabolism, Itaconate formation in RC4' required de novo protein biosynthesis. Krebs cycle intermediates increased in both strains during the production of itaconic acid. The Embden-Meyerhof-Parnas pathway and the Krebs cycle were shown to be involved in this biosynthesis by using C-14- and C-13-labelled substrates and nuclear magnetic resonance spectroscopy. A metabolic pathway for itaconate formation from glucose in A, terreus is proposed.

引用

页码：3573 / 3578

页数：6

共 22 条

[1] CIS-ACONITIC DECARBOXYLASE [J].

BENTLEY, R ;

THIESSEN, CP .

SCIENCE, 1955, 122 (3164) :330-330

[2]

BENTLEY R, 1957, J BIOL CHEM, V226, P689

[3]

BENTLEY R, 1957, J BIOL CHEM, V226, P703

[4]

BENTLEY R, 1957, J BIOL CHEM, V226, P673

[5]

Bentley R, 1956, AM SOC BIOL CHEM, V15, P219

[6]

Billington R. H, 1969, CHEM PROCESS, V15, P8

[7] Studies in the biochemistry of micro-organisms XIII. Itaconic acid, a metabolic product of a strain of Aspergillus terreus Thom [J].

Calam, CT ;

Oxford, AE ;

Raistrick, H .

BIOCHEMICAL JOURNAL, 1939, 33 :1488-1495

[8]

CROS P, 1988, Patent No. 8805487

[9] FLUORIDE EFFECTS ON P-31 NMR-SPECTRA OF MACROPHAGES [J].

DUCROCQ, C ;

LENFANT, M ;

WERNER, GH ;

GILLET, B ;

BELOEIL, JC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 147 (02) :519-525

[10] THE SUBCELLULAR ORGANIZATION OF ITACONATE BIOSYNTHESIS IN ASPERGILLUS-TERREUS [J].

JAKLITSCH, WM ;

KUBICEK, CP ;

SCRUTTON, MC .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :533-539

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