LIMITATIONS OF GRAPHICAL ANALYSIS OF ELUTION CURVES

被引:4
作者
HAGEMEIJER, F
VANREMOO.P
机构
[1] Laboratoire de Biochimie, Département, Clinique et de Pathologie Médicales
[2] Chaire d'Electricité, Ecole Royale Militaire, Bruxelles
关键词
D O I
10.1016/S0022-5193(69)80062-7
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
With the use of an analogue computer, elution curves have been simulated from theoretical mathematical models. These curves are submitted to the graphical analysis currently used. Differences between theoretical and measured data correspond essentially to the fact that back-diffusion is overlooked. This can be corrected with Huxley's equations whenever only two compartments actually exchange with the washout solution. When more than two compartments can be individualized, the measured contents of these compartments are good approximations of the actual contents whenever the time constants of exchange are very different. On the other hand, the measured time constants are only of the same order of magnitude as the actual time constants and result from the interrelations between outflux and back-diffusion. The analogue computer should be used to simulate elution curves in order to verify the mathematical model for biological preparations in washout experiments. © 1969 Academic Press Inc. (London) Ltd.
引用
收藏
页码:236 / +
页数:1
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