TYPE-1 FIMBRIAE MUTANTS OF ESCHERICHIA-COLI K12 - CHARACTERIZATION OF RECOGNIZED AFIMBRIATE STRAINS AND CONSTRUCTION OF NEW FIM DELETION MUTANTS

被引：143

作者：

BLOMFIELD, IC ^{[1
]}

MCCLAIN, MS ^{[1
]}

EISENSTEIN, BI ^{[1
]}

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,6643 MED SCI BLDG,ANN ARBOR,MI 48109

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00790.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used Southern hybridization analysis to characterize the extent of fim homology in recognized type 1 fimbriae mutants of Escherichia coli K12, including strains HB101, P678-54, and VL584. We have found extensive homology in strain HB101, and confirm that P678-54 lacks the majority of fim DNA. Strain VL584 contains a deletion of the entire fim region. We have used a new allelic exchange procedure to generate novel fim deletion derivatives of strains MG1655, MM294, and YMC9. To increase the utility of the new deletion strains we also isolated recA derivatives of each mutant. These strains facilitate the isolation, characterization, and manipulation of cloned fimbriae genes from diverse sources.

引用

页码：1439 / 1445

页数：7

共 31 条

[1] AN INVERTIBLE ELEMENT OF DNA CONTROLS PHASE VARIATION OF TYPE-1 FIMBRIAE OF ESCHERICHIA-COLI [J].

ABRAHAM, JM ;

FREITAG, CS ;

CLEMENTS, JR ;

EISENSTEIN, BI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (17) :5724-5727

[2]

ALDER HI, 1967, P NATL ACAD SCI USA, V57, P321

[3] PHYSICAL AND GENETIC-CHARACTERIZATION OF THE GLNA-GLNG REGION OF THE ESCHERICHIA-COLI CHROMOSOME [J].

BACKMAN, K ;

CHEN, YM ;

MAGASANIK, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3743-3747

[4] ALLELIC EXCHANGE IN ESCHERICHIA-COLI USING THE BACILLUS-SUBTILIS SACB GENE AND A TEMPERATURE-SENSITIVE PSC101 REPLICON [J].

BLOMFIELD, IC ;

VAUGHN, V ;

REST, RF ;

EISENSTEIN, BI .

MOLECULAR MICROBIOLOGY, 1991, 5 (06) :1447-1457

[5] POSITIVE SELECTION FOR LOSS OF TETRACYCLINE RESISTANCE [J].

BOCHNER, BR ;

HUANG, HC ;

SCHIEVEN, GL ;

AMES, BN .

JOURNAL OF BACTERIOLOGY, 1980, 143 (02) :926-933

[6] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[7] COMPLEMENTATION ANALYSES OF RECOMBINANT PLASMIDS ENCODING TYPE-1 FIMBRIAE OF MEMBERS OF THE FAMILY ENTEROBACTERIACEAE [J].

CLEGG, S ;

PRUCKLER, J ;

PURCELL, BK .

INFECTION AND IMMUNITY, 1985, 50 (01) :338-340

[8] CLONING OF GENES DETERMINING THE PRODUCTION OF MANNOSE-RESISTANT FIMBRIAE IN A UROPATHOGENIC STRAIN OF ESCHERICHIA-COLI BELONGING TO SEROGROUP-O6 [J].

CLEGG, S .

INFECTION AND IMMUNITY, 1982, 38 (02) :739-744

[9]

CSONKA LN, 1979, GENETICS, V93, P321

[10] FLU, A METASTABLE GENE CONTROLLING SURFACE-PROPERTIES OF ESCHERICHIA-COLI [J].

DIDERICHSEN, B .

JOURNAL OF BACTERIOLOGY, 1980, 141 (02) :858-867

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