EXPERIMENTAL AND THEORETICAL EVIDENCE FOR CONVECTIVE NUTRIENT TRANSPORT IN AN IMMOBILIZED CELL SUPPORT

被引:12
作者
BRINGI, V [1 ]
DALE, BE [1 ]
机构
[1] COLORADO STATE UNIV,DEPT AGR & CHEM ENGN,FT COLLINS,CO 80523
关键词
D O I
10.1021/bp00003a008
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Even though immobilized‐cell reactors possess several engineering advantages over free‐cell reactors, their full potential has not been realized because mass transfer often limits the rate of nutrient supply and product removal from immobilized cell supports. We studied the interaction between mass transfer and reaction kinetics in the anaerobic conversion of glucose to CO2 and ethanol by yeast immobilized in a porous rotating disk on the agitator shaft of a conventional CSTR. A Sherwood number correlation was used to show that external mass‐transfer resistances were negligible under typical operating conditions. The modulus of Weisz based on observable reaction parameters was used to gauge the importance of pore diffusion limitations. Under conditions for which significant pore diffusion effects and hence low effectiveness factors (η =ca. 0.1) would be predicted, the observed reaction rates were much higher than expected (η=ca. 1), suggesting that pore diffusion limitations were at least partially relieved by convective transport of glucose into the support. Two possible mechanisms of convective transport are discussed. We hypothesize that gas evolution was responsible for the convective enhancement of glucose supply. Copyright © 1990 American Institute of Chemical Engineers (AIChE)
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页码:205 / 209
页数:5
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