INTERACTION OF THE ERYTHROID TRANSCRIPTION FACTOR CGATA-1 WITH A CRITICAL AUTO-REGULATORY ELEMENT

被引：37

作者：

SCHWARTZBAUER, G ^{[1
]}

SCHLESINGER, K ^{[1
]}

EVANS, T ^{[1
]}

机构：

[1] UNIV PITTSBURGH,DEPT BIOL SCI,LANGLEY HALL,ROOM 518,PITTSBURGH,PA 15260

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 17期

关键词：

D O I：

10.1093/nar/20.17.4429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have performed a mutational analysis of the promoter for the chicken erythroid-specific GATA-1 transcription factor, and have investigated in detail the interaction of the factor with an upstream auto-regulatory element (ARE). We find that a single proximal GATA binding site of the ARE is required for promoter activity in primary erythroid cells; however, this minimal promoter is inappropriately active in fibroblasts. At least two molecules of GATA-1 can interact with the ARE, and sequences outside of the consensus site appear critical for the transcriptional activity of the bound protein. Finally, we provide evidence for complex protein/DNA interactions at the ARE, including the ability of GATA-1 to bend DNA.

引用

页码：4429 / 4436

页数：8

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