YEAST COATOMER CONTAINS A SUBUNIT HOMOLOGOUS TO MAMMALIAN BETA'-COP

被引：20

作者：

HARTER, C ^{[1
]}

DRAKEN, E ^{[1
]}

LOTTSPEICH, F ^{[1
]}

WIELAND, FT ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,GENZENTRUM,D-82152 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

SECRETORY PATHWAY; TRANSPORT VESICLE; COATOMER; YEAST HOMOLOG;

D O I：

10.1016/0014-5793(93)80487-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homologue of the mammalian coatomer complex was isolated from yeast cytosol and separated on a modified urea-containing SDS-polyacrylamide gel system. An additional band in the 100 kDa molecular weight range appeared when compared to the protein pattern obtained in conventional Laemmli gels, exactly as observed for mammalian coatomer. Cross-reactivity with an anti-peptide antibody raised against the C-terminus of beta'-COP from bovine, and N-terminal sequence analysis, revealed that this protein from yeast is related to beta'-COP from mammals.

引用

页码：71 / 73

页数：3

共 24 条

[1] AHLE S, 1989, J BIOL CHEM, V264, P20089
[2] THE PRODUCT OF THE PRP4 GENE OF S CEREVISIAE SHOWS HOMOLOGY TO BETA-SUBUNITS OF G-PROTEINS
DALRYMPLE, MA
PETERSENBJORN, S
FRIESEN, JD
BEGGS, JD
[J]. CELL, 1989, 58 (05) : 811 - 812
[3] COP1, AN ARABIDOPSIS REGULATORY GENE, ENCODES A PROTEIN WITH BOTH A ZINC-BINDING MOTIF AND A G-BETA HOMOLOGOUS DOMAIN
DENG, XW
MATSUI, M
WEI, N
WAGNER, D
CHU, AM
FELDMANN, KA
QUAIL, PH
[J]. CELL, 1992, 71 (05) : 791 - 801
[4] BINDING OF ARF AND BETA-COP TO GOLGI MEMBRANES - POSSIBLE REGULATION BY A TRIMERIC G-PROTEIN
DONALDSON, JG
KAHN, RA
LIPPINCOTTSCHWARTZ, J
KLAUSNER, RD
[J]. SCIENCE, 1991, 254 (5035) : 1197 - 1199
[5] A NEW SILICONIZED-GLASS FIBER AS SUPPORT FOR PROTEIN-CHEMICAL ANALYSIS OF ELECTROBLOTTED PROTEINS
ECKERSKORN, C
MEWES, W
GORETZKI, H
LOTTSPEICH, F
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (03): : 509 - 519
[6] ELAZAR Z, 1993, UNPUB
[7] HARAKUGE S, UNPUB
[8] SEC21 IS A GENE REQUIRED FOR ER TO GOLGI PROTEIN-TRANSPORT THAT ENCODES A SUBUNIT OF A YEAST COATOMER
HOSOBUCHI, M
KREIS, T
SCHEKMAN, R
[J]. NATURE, 1992, 360 (6404) : 603 - 605
[9] Iniguez-Lluhi Jorge, 1993, Trends in Cell Biology, V3, P230, DOI 10.1016/0962-8924(93)90122-H
[10] MICROINJECTED ANTIBODIES AGAINST THE CYTOPLASMIC DOMAIN OF VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN BLOCK ITS TRANSPORT TO THE CELL-SURFACE
KREIS, TE
[J]. EMBO JOURNAL, 1986, 5 (05) : 931 - 941

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