ISOLATION AND CHARACTERIZATION OF TROPOMYOSIN FROM FISH MUSCLE

被引：32

作者：

HEELEY, DH

HONG, C

机构：

[1] Department of Biochemistry, Memorial University, St John's

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 108卷 / 01期

关键词：

TROPOMYOSIN; FISH MUSCLE; CLUPEA HARENGUS; SALVELINUS ALPINUS; SALMO SALAR; GADUS MORHUA; MELANOGRAMMUS AEGLEFINUS; UROPHYCIS; POLLACHIUS VIRENS; SEBASTES; HIPPOGLOSSUS HIPPOGLOSSUS; PSEUDOPLEURONECTES AMERICANUS; XIPHIAS GLADIUS; ANARCHICHAS LUPUS; ISURUS OXYRINCHUS; SCOMBER SCROBRUS; PRIONACE GLAUCA; RAJA RADIATA; PETROMYZON MARINUS;

D O I：

10.1016/0305-0491(94)90169-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comprehensive survey of tropomyosin from various fish myotomal muscles is reported. The fish tropomyosins were blocked at the N-terminus and, as expected, were found to be of similar amino acid composition, alpha-helical content (> 90% at 10 degrees C) and molecular weight to other vertebrate striated muscle forms. The tropomyosins of salmonids and herring muscle were noticeably heterogeneous when assessed by 2D-PAGE. The distribution of isoforms was tissue-specific: slow muscle contained alpha-type tropomyosin while fast muscle contained beta-type tropomyosin. In other species (cod, haddock, wolf-fish and sharks) alpha-type tropomyosins were present in both kinds of muscle but beta-tropomyosin was absent.

引用

页码：95 / 106

页数：12

共 30 条

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[2]

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[3]

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