IMPROVED DETECTION OF ORAL SPIROCHETES WITH AN ANAEROBIC CULTURE METHOD

被引：20

作者：

UMEDA, M ^{[1
]}

ISHIKAWA, I ^{[1
]}

BENNO, Y ^{[1
]}

MITSUOKA, T ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV,SCH DENT,DEPT PERIODONTOL,YUSHIMA,BUNKYO KU,TOKYO 113,JAPAN

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 02期

关键词：

anaerobic method; Medium; 10; oral spirochetes; plate‐in‐bottle” method;

D O I：

10.1111/j.1399-302X.1990.tb00234.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A method for routine culturing of oral spirochetes was studied in periodontitis patients and subjects with healthy gingivae. Subgingival plaque bacteria, including oral spirochetes were cultured anaerobically using the steel wool jar method, (he glove‐box procedure and the plate‐in‐bottle method. They were also observed directly with a phase‐contrast microscope. The number of colonies of recovered spirochetes was significantly higher with the plate‐in‐bottle method when Medium 10 (M10) or M10 supplemented with 10% rumen fluid was used. In almost all cases, spirochetes were detected with this culture method. The ratios of spirochetes from patient samples, cultured by the plate‐in‐bottle method, and of other microorganisms, cultured in Eggerth‐Gagnon (EG) medium by the steel wool jar method, were almost identical of those observed by phase‐contrast microscopy. These results indicate that it can be just as possible to culture certain oral spirochetes as other anaerobic bacteria. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：90 / 94

页数：5

共 23 条

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