DIABETES-INDUCED DECREASE IN THE MESSENGER-RNA CODING FOR SARCOPLASMIC-RETICULUM CA2+-ATPASE IN ADULT-RAT CARDIOMYOCYTES

被引：38

作者：

RUSS, M ^{[1
]}

REINAUER, H ^{[1
]}

ECKEL, J ^{[1
]}

机构：

[1] DIABET FORSCHUNGSINST, MOLEC CARDIOL LAB, W-4000 DUSSELDORF 1, GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90977-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study examined the level of the mRNA coding for the sarcoplasmic reticulum (SR) Ca2+-ATPase in isolated ventricular myocytes from streptozotocin-diabetic rats and genetically obese (fa/fa) rats using Northern blotting techniques. In both animal models one single transcript with a mean size of 4.12 kb could be detected. In insulin-deficient rats the Ca2+-ATPase transcript level decreased by 51% when compared to normal rats. In obese animals a modest decrease in the Ca2+-ATPase mRNA content to 77% of lean controls has been detected. Decreased mRNA expression of the SR Ca2+-ATPase may partly explain the delayed diastolic relaxation observed in the diabetic heart. © 1991.

引用

页码：906 / 912

页数：7

共 25 条

[1] INFLUENCE OF VERAPAMIL ON SOME SUBCELLULAR DEFECTS IN DIABETIC CARDIOMYOPATHY [J].

AFZAL, N ;

PIERCE, GN ;

ELIMBAN, V ;

BEAMISH, RE ;

DHALLA, NS .

AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 256 (04) :E453-E458

[2] CA INFLUX AND SARCOPLASMIC-RETICULUM CA RELEASE IN CARDIAC-MUSCLE ACTIVATION DURING POSTREST RECOVERY [J].

BERS, DM .

AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 248 (03) :H366-H381

[3] INFLUENCE OF EXPERIMENTAL DIABETES ON SARCOPLASMIC-RETICULUM FUNCTION IN RAT VENTRICULAR MUSCLE [J].

BOUCHARD, RA ;

BOSE, D .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02) :H341-H354

[4] 2 CA-2+ ATPASE GENES - HOMOLOGIES AND MECHANISTIC IMPLICATIONS OF DEDUCED AMINO-ACID-SEQUENCES [J].

BRANDL, CJ ;

GREEN, NM ;

KORCZAK, B ;

MACLENNAN, DH .

CELL, 1986, 44 (04) :597-607

[5]

BRANDL CJ, 1987, J BIOL CHEM, V262, P3768

[6]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[7] FUNCTION OF THE SARCOPLASMIC-RETICULUM AND EXPRESSION OF ITS CA-2+-ATPASE GENE IN PRESSURE OVERLOAD-INDUCED CARDIAC-HYPERTROPHY IN THE RAT [J].

DELABASTIE, D ;

LEVITSKY, D ;

RAPPAPORT, L ;

MERCADIER, JJ ;

MAROTTE, F ;

WISNEWSKY, C ;

BROVKOVICH, V ;

SCHWARTZ, K ;

LOMPRE, AM .

CIRCULATION RESEARCH, 1990, 66 (02) :554-564

[8] (CA-2+ + MG-2+)-DEPENDENT ATPASE MESSENGER-RNA FROM SMOOTH-MUSCLE SARCOPLASMIC-RETICULUM DIFFERS FROM THAT IN CARDIAC AND FAST SKELETAL-MUSCLES [J].

DELABASTIE, D ;

WISNEWSKY, C ;

SCHWARTZ, K ;

LOMPRE, AM .

FEBS LETTERS, 1988, 229 (01) :45-48

[9] INSULIN RESISTANCE IN THE HEART - STUDIES ON ISOLATED CARDIOCYTES OF GENETICALLY-OBESE ZUCKER RATS [J].

ECKEL, J ;

WIRDEIER, A ;

HERBERG, L ;

REINAUER, H .

ENDOCRINOLOGY, 1985, 116 (04) :1529-1534

[10] G-PROTEIN-MEDIATED REGULATION OF THE INSULIN-RESPONSIVE GLUCOSE TRANSPORTER IN ISOLATED CARDIAC MYOCYTES [J].

ECKEL, J ;

GERLACHESKUCHEN, E ;

REINAUER, H .

BIOCHEMICAL JOURNAL, 1990, 272 (03) :691-696

← 1 2 3 →