DIFFERENTIAL DETERMINATION PROCEDURE FOR SPERMINE AND SPERMIDINE WITH BEEF PLASMA AMINE OXIDASE

被引:12
作者
YAMADA, H [1 ]
ISOBE, K [1 ]
TANI, Y [1 ]
HIROMI, K [1 ]
机构
[1] KYOTO UNIV,DEPT FOOD SCI & TECHNOL,KYOTO 606,JAPAN
来源
AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1979年 / 43卷 / 12期
关键词
D O I
10.1080/00021369.1979.10863836
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
An enzymatic procedure for the differential determination of polyamines, spermine and spermidine, has been established using beef plasma amine oxidase. This method was specific for these polyamines and required only one reaction system. Small amounts of polyamines (10 μM to 80 μM of spermine and 10 μM to 100μM of spermidine) were assayed by solving two simultaneous equations obtained from the rate assay method and the end point assay method. The calculated values were in good agreement with those obtained by other method. © 1979 Taylor & Francis Group, LLC.
引用
收藏
页码:2487 / 2491
页数:5
相关论文
共 20 条
  • [1] ENZYMIC ASSAY FOR SPERMINE AND SPERMIDINE
    BACHRACH, U
    RECHES, B
    [J]. ANALYTICAL BIOCHEMISTRY, 1966, 17 (01) : 38 - &
  • [2] DENTON MD, 1973, CLIN CHEM, V19, P905
  • [3] FUJITA K, 1976, CANCER RES, V36, P1320
  • [4] LIPTON A, 1975, CANCER, V35, P464, DOI 10.1002/1097-0142(197502)35:2<464::AID-CNCR2820350225>3.0.CO
  • [5] 2-8
  • [6] MARTON LJ, 1973, CLIN CHEM, V19, P923
  • [7] MIYASHITA Y, 1975, Patent No. 509492
  • [8] RAINA A, 1975, MED BIOL, V53, P121
  • [9] RUSSELL DH, 1977, CLIN CHEM, V23, P22
  • [10] RUSSELL DH, 1975, CLIN CHEM, V21, P860