ROLE OF INSULIN IN THE REGULATION OF STEARIC-ACID DESATURASE ACTIVITY IN LIVER AND ADIPOSE-TISSUE FROM OBESE-HYPERGLYCEMIC (OB-OB) AND LEAN MICE

The relationship between the hyperinsulinaemia of obese-hyperglycaemic (ob/ob) mice and their high activity of stearic acid Δ9-desaturase compared with lean mice has been investigated. The concentrations of plasma insulin in obese mice were decreased by 71, 88 and 96% after treatment either with alloxan or food restriction to maintain the same weight as lean mice, or treatment of the weight restricted mice with alloxan followed by feeding ad libitum. The concentration of plasma insulin produced by the latter treatment was the same as in normal lean mice. After treatment the hepatic desaturase activities were 24, 68 and 19% less respectively on a cell basis than in livers from untreated obese mice, and the total epididymal fat-pad activities were lower by 16, 62 and 57%. These results suggest that hyperinsulinaemia is not essential for the increased hepatic desaturase, but may be important in adipose tissue. Food intake appears to be a significant factor controlling the hepatic desaturase activity, but even this may be subject to overriding regulation by the concentration of esterified linoleic acid in the liver lipids, which was negatively correlated (r=0.91, P<0.001) with desaturase activity.