DEVELOPMENT OF ACETYLCHOLINE SENSOR USING CARBON-FIBER (AMPEROMETRIC DETERMINATION)

被引：50

作者：

NAVERA, EN ^{[1
]}

SODE, K ^{[1
]}

TAMIYA, E ^{[1
]}

KARUBE, I ^{[1
]}

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,4-6-1 KOMABA,MEGURO KU,TOKYO 153,JAPAN

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 08期

关键词：

CARBON FIBER; CO-IMMOBILIZED ENZYMES; PVA-SBQ; AMPEROMETRIC DETECTION;

D O I：

10.1016/0956-5663(91)87021-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An enzyme sensor is developed using carbon fiber to measure acetylcholine concentration. The mechanism is based on the detection of H2O2 which is a product of the sequential enzyme reactions of acetylcholinesterase and choline oxidase. The fabrication of the electrode is described. The sensor is polarized at 1.2 V. Enzymes are co-immobilized in polyvinyl alcohol containing styryl pyrydinium (photo-crosslinkable polymer). A fast response time of 0.8 min is obtained. A linear correlation is observed between 0.2 and 1.0 mM. Other optimal operational conditions with respect to pH, temperature and stability are discussed. The use of carbon fiber containing co-immobilized enzymes could offer several novel advantages especially in neuroscience research. In conclusion, the aims of the present work are centered on carbon fiber electrode fabrication, immobilization and electrochemical measurements.

引用

页码：675 / 680

页数：6

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