A RAPID TECHNIQUE FOR THE ISOLATION OF DNA FROM CLINICAL ISOLATES OF CANDIDA-ALBICANS

被引：3

作者：

MATHABA, LT ^{[1
]}

FRANKLYN, KM ^{[1
]}

WARMINGTON, JR ^{[1
]}

机构：

[1] CURTIN UNIV TECHNOL,SCH BIOMED SCI,BENTLEY,WA 6102,AUSTRALIA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1993年 / 17卷 / 01期

基金：

英国医学研究理事会;

关键词：

CANDIDA-ALBICANS; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; FINGERPRINTING; ELECTROPHORETIC KARYOTYPING;

D O I：

10.1016/0167-7012(93)90075-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have modified the method originally developed by Schwartz and Cantor [1] for the extraction of intact yeast chromosomal DNA for pulsed field gel electrophoresis. The modified method allows clinical isolates of Candida albicans to be cultured and the DNA extracted in a 24- or 96-well microtitre tray. The DNA is of sufficient quality to enable the isolates to be electrophoretically karyotyped by pulsed field gel electrophoresis, or digested with a restriction endonuclease for subsequent restriction fragment length polymorphism analysis. Several microtitre trays can be processed at one time. This makes it possible to prepare DNA from hundreds of clinical isolates, rapidly and with a minimum amount of labor. Further, it would be relatively easy to adapt this method to enable the extraction of DNA from any type of cell, prokaryotic or eukaryotic.

引用

页码：17 / 25

页数：9

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