QUANTITATION OF ZEARALENONE BY GAS-LIQUID-CHROMATOGRAPHY ON CAPILLARY GLASS-COLUMNS

被引:21
作者
THOUVENOT, DR
MORFIN, RF
机构
[1] Laboratoire de Biochimie, Faculté des Sciences
来源
JOURNAL OF CHROMATOGRAPHY | 1979年 / 170卷 / 01期
关键词
D O I
10.1016/S0021-9673(00)84248-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A procedure is described for the quantitation of zearalenone from corn by gas-liquid chromatography. An internal standard, zearalanone, is first mixed with the finely ground corn, followed by extraction with ethyl acetate, purification by thin-layer chromatography and formation of trimethyl silyl ether (TMS) and methyl oxime (MOX) derivatives. Positive identification of zearalenone is based upon the retention times of the TMS and MOX-TMS derivatives being identical with those of the standards, comparison of chromatograms from both derivatives and characteristic twin peaks of zearalenone MOX-TMS. The detection limit of 100 parts per 109 zearalenone in corn could only be improved by modification of the described procedure. © 1979.
引用
收藏
页码:165 / 173
页数:9
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